RIPE PAPAYA (CARICA PAPAYA) REDUCES UTERINE LENGTH AND WEIGHT IN MICE (MUS MUSCULUS L)
DOI:
https://doi.org/10.55197/qjmhs.v4i3.145Keywords:
ripe papaya juice, papain, uterus, fetalAbstract
Unripe or green papaya is one of the foods that should be avoided during pregnancy due to its potential to cause fetal abnormalities and/or miscarriage. Papaya contains papain, polysaccharides, alkaloids, saponins, and flavonoids, which are suspected to interfere with the hypothalamic-pituitary axis, disrupting GnRH secretion and subsequently affecting follicular formation, development, and maturation. This study aimed to investigate the effect of ripe papaya (Carica papaya) fruit juice administration on uterine characteristics in mice (Mus musculus). A total of 12 adult female mice were randomly divided into four groups and administered ripe papaya juice orally (gavage) at doses of 0% (control), 20%, 40%, and 80% for ten consecutive days. Following treatment, the mice were sacrificed for uterine collection. Data were analyzed using ANOVA followed by Duncans post hoc test. The results indicated that the administration of ripe papaya juice significantly reduced both left and right uterine lengths and lean wet weights at all doses. The reduction in uterine length on both sides suggests that active compounds in papaya, such as the enzyme papain, may influence cellular proliferation processes within uterine tissue.
References
[1] Adebiyi, A., Adaikan, P.G., Prasad, R.N.V. (2002): Papaya (Carica papaya) consumption is unsafe in pregnancy: fact or fable? Scientific evaluation of a common belief in some parts of Asia using a rat model. – British Journal of Nutrition 88(2): 199-203.
[2] Adigwe, C.C., Oremosu, A.A., Gbotolorun, S.C., Kuseiju, T.O., Akang, E.N., Noronha, C.C., Okalanwon, A.O. (2012): Effects of the alcoholic root extract of the male Carica papaya on ovulation, gestation and fetal parameters in female Sprague-Dawley rats. – Journal of Medicinal Plants Research 6(16): 3093-3097.
[3] Al-Ikhsan, A.H., Candra, A., Rahmad, R. (2024): Pengaruh Buah Pepaya (Carica papaya L.) Terhadap Kekuatan Otot, Vital Sign Dan Glukosa. – Jurnal Ilmu Kedokteran dan Kesehatan 11(12): 2387-2396.
[4] Annaházi, A., Schröder, A., Schemann, M. (2021): Region‐specific effects of the cysteine protease papain on gastric motility. Neurogastroenterology & Motility 33(7): 10p.
[5] Anuar, N.S., Zahari, S.S., Taib, I.A., Rahman, M.T. (2008): Effect of green and ripe Carica papaya epicarp extracts on wound healing and during pregnancy. – Food and Chemical Toxicology 46(7): 2384-2389.
[6] Auta, T., Hassan, A.T. (2016): Alteration in oestrus cycle and implantation in Mus musculus administered aqueous wood ash extract of Azadirachta indica (neem). – Asian Pacific Journal of Reproduction 5(3): 188-192.
[7] Babalola, B.A., Akinwande, A.I., Otunba, A.A., Adebami, G.E., Babalola, O., Nwufo, C. (2024): Therapeutic benefits of Carica papaya: A review on its pharmacological activities and characterization of papain. – Arabian Journal of Chemistry 17(1): 11p.
[8] Byers, S.L., Wiles, M.V., Dunn, S.L., Taft, R.A. (2012): Mouse estrous cycle identification tool and images. – PloS One 7(4): 5p.
[9] Campos, M.M., Cabral, K.S., Nunes, P.C.R., Estevam, A.A.V., Bianco, B.T., Alves, B.B.L., da Silva Ventura, G., de Oliveira Santana, R., da Silva, N.M.F., Lopes, L.H., Mendes, F.A. (2023): Embryotoxic, teratogenic and abortive effects caused by the consumption of plants for food and medicinal use. – Revista Presença 9(20): 152-217.
[10] Candra, A., Fahrimal, Y., Yusni, Y., Azwar, A., Santi, T. D. (2024): Phytochemistry and antifatigue activities of Carica papaya leaf from geothermal, coastal and urban areas, Indonesia. – Narra J 4(1): 9p.
[11] Choudhary, R., Kaushik, R., Chawla, P., Manna, S. (2025): Exploring the extraction, functional properties, and industrial applications of papain from Carica papaya. – Journal of the Science of Food and Agriculture 105(3): 1533-1545.
[12] David Troncoso, F., Alberto Sánchez, D., Luján Ferreira, M. (2022): Production of plant proteases and new biotechnological applications: an updated review. – ChemistryOpen 11(3): 38p.
[13] Gulia, N., Bisla, A., Honparkhe, M. (2023): A review of applications and scope of intrauterine proteolytic enzymes therapy for treatment of uterine infections in dairy animals. – International Journal of Veterinary Sciences and Animal Husbandry 8: 66-71.
[14] Harlita, H., Probosari, R.M., Ariyanto, J. (2015): Perubahan histologis uterus tikus putih (Rattus norvegicus) galur Wistar: Aktifitas antifertilitas ekstrak kulit biji mete (Anacardium occidentale L.). – Bioedukasi: Jurnal Pendidikan Biologi 8(2): 1-4.
[15] Hidayati, N., Ruhayani, W. (2021): Pengaruh Kombinasi Jus Buah Pepaya (Carica papaya L) Dan Sari Daun Katuk (Sauropus androgynus) Pada Pembuatan Permen Jelly Terhadap Organoleptik, Kandungan Zat Besi Dan Vitamin C. – Infokes 11(2): 515-521.
[16] Iipinge, L.N. (2019): Effect of pawpaw (Carica Papaya) seed meal on growth performance, feed utilization, survival and masculinization of sexualy undifferentiated three spotted Tilapia (Oreochromis Andersonii) fry. – University of Namibia 216p.
[17] Jamal, G.A., Jahangirian, E., Hamblin, M.R., Mirzaei, H., Tarrahimofrad, H., Alikowsarzadeh, N. (2025): Proteases, a powerful biochemical tool in the service of medicine, clinical and pharmaceutical. – Preparative Biochemistry & Biotechnology 55(1): 1-25.
[18] Jaiswal, P., Kumar, P., Singh, V.K., Singh, D.K. (2010): Carica papaya Linn: A potential source for various health problems. – Journal of Pharmacy Research 3(5): 998-1003.
[19] Jiménez, V.M., Mora-Newcomer, E., Gutiérrez-Soto, M.V. (2014): Biology of the papaya plant. – Genetics and Genomics of Papaya 16p.
[20] Kusemiju, T.O. (2008): Contraceptive and Morphometric Effects of the Aqueous Extract of Carica Papaya Bark on Male Sprague Dawley Rats. – University of Lagos (Nigeria) 208p.
[21] Koul, B., Pudhuvai, B., Sharma, C., Kumar, A., Sharma, V., Yadav, D., Jin, J.O. (2022): Carica papaya L.: a tropical fruit with benefits beyond the tropics. – Diversity 14(8): 33p.
[22] Malek, K., Norazan, M., Ramaness, P., Othman, N.Z., Malek, R., Aziz, R., Aladdin, A., El Enshasy, H. (2016): Cysteine Proteases from Carica papaya: An important enzyme group of many industrial applications. – IOSR Journal of Pharmacy and Biological Sciences 11(2): 11-16.
[23] Maliha, M., Rashid, T.U., Rahman, M.M. (2024): A green strategy for collagen extraction from tannery raw trimmings using papain enzyme: Process optimization by MW-TOPSIS for enhanced yield. – International Journal of Biological Macromolecules 262: 13p.
[24] Marwah, M., Tawali, A.B., Latif, R. (2023): The effect of proteolytic enzymes from the papain of papaya sap (Cacica papaya L.) and the streplin of serut sap (Streblus asper L.) with different temperatures of enzyme addition in making Dangke. – In AIP Conference Proceedings, AIP Publishing 2596: 15p.
[25] Memudu, A.E., Oluwole, T.J. (2021): The contraceptive potential of Carica papaya seed on oestrus cycle, progesterone, and histomorphology of the Utero-ovarian tissue of adult wistar rats. – JBRA Assisted Reproduction 25(1): 10p.
[26] Nekoueinaeini, S.M., Aliahmadi, A., Soleimani, N. (2024): An Overview of Papain Enzyme Characteristics, Applications and Production. – Plant, Algae, and Environment 8(2): 1505-1527.
[27] Novinec, M., Lenarčič, B. (2013): Papain-like peptidases: structure, function, and evolution. – Biomolecular Concepts 4(3): 287-308.
[28] Parera, N., Priyo, B., Rizqiati, H., Felya, O. (2022): Pengaruh jenis penstabil dan perbandingan pepaya dengan sari kurma terhadap karakteristik sorbet pepaya (Carica papaya. L). – Jurnal Teknologi Pangan 2(1): 40-45.
[29] Praveena, P., Jethinlalkhosh, J.P., Doss, V.A. (2017): Evaluation of Uterotonic Activity of Hydro-ethanolic Extract of Unripe Fruit of Carica papaya Linn using Wistar Albino Rats. Indian Journal of Pharmaceutical Education & Research 51(4s) S615-S622.
[30] Ruijtenberg, S., Van Den Heuvel, S. (2016): Coordinating cell proliferation and differentiation: Antagonism between cell cycle regulators and cell type-specific gene expression. – Cell Cycle 15(2): 196-212.
[31] Santi, T.D., Zakaria, R., Candra, A., Nauval, M.D. (2023): Analysis active compounds of Carica papaya, Averrhoa bilimbi, and Chromolaena odorata leaves from geothermal area. – In AIP Conference Proceedings, AIP Publishing 2583: 10p.
[32] Stanciu, M., Sassville, D. (2015): Contact urticaria, dermatitis and respiratory allergy caused by enzymes. Contact urticaria syndrome. – Florida: Taylor & Francis Group 19p.
[33] Suryani, D.R., Harlita, H. (2025): Pengaruh Konsumsi Pepaya (Carica papaya) Muda terhadap Perkembangan Fetus Mencit (Mus musculus L.). – Jurnal Sains dan Edukasi Sains 8(1): 13-19.
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