ALKALOID AND FLAVONOID CONTENT OF PTEROCARPUS SANTALINOIDES: EFFECTS ON PANCREATIC BIOCHEMISTRY IN MICE
DOI:
https://doi.org/10.55197/qjmhs.v5i1.201Keywords:
caerulein-induced pancreatitis, alkaloids, flavonoids, pancreatic biochemistry, oxidative stress, Pterocarpus SantalinoidesAbstract
Pancreatitis is a disorder caused by oxidative and inflammatory damage to pancreatic tissues, leading to biochemical and functional abnormalities. Phytochemicals, including alkaloids and flavonoids, are acknowledged for their antioxidant and anti-inflammatory properties. This study aims to analyse the total alkaloid and total flavonoid concentrations in the leaves, stems, and roots of Pterocarpus santalinoides (Nturukpa) and assess their effects on pancreatic biochemical indicators in mice with caerulein-induced pancreatitis. The hydroethanolic extracts of the plant parts were quantitatively analysed for alkaloids and flavonoids. Acute pancreatitis was induced in mice with consecutive caerulein injections. The extracts were administered orally for a period of 21 days. The levels of pancreatic insulin, antioxidant enzyme activity, lipid peroxidation, and inflammatory markers were evaluated. The stem extracts demonstrated the greatest quantities of alkaloids and flavonoids, followed by the leaf extracts, while the root extracts shown much lower levels. The administration of caerulein caused considerable oxidative stress, inflammation, and a reduction in pancreatic insulin levels. Treatment with the standard drug (ocreotide) and stem and leaf extracts significantly improved antioxidant enzyme activity, reduced lipid peroxidation and inflammation, and increased insulin levels (p < 0.05). P. santalinoides, particularly its stem and leaf fractions, mitigates caerulein-induced pancreatic biochemical disruptions via alkaloid- and flavonoid-mediated antioxidant and anti-inflammatory mechanisms.
References
[1] Adhikari, B. (2021): Roles of alkaloids from medicinal plants in the management of diabetes mellitus. – Journal of Chemistry 10p.
[2] Agu, P.C., Ezeh, E.M., Nwosu, C.I. (2023): Characterization of nutritional and phytochemical compositions of locally consumed leafy vegetables from Afikpo, Ebonyi State, Nigeria. – Caritas Journal of Physical and Life Science 1(1): 8p.
[3] Aparna, B., Hema, B.P. (2022): Preliminary screening and quantification of flavonoids in selected seeds of Apiaceae by UV-visible spectrophotometry with evaluation study on different aluminium chloride complexation reaction. – Indian Journal of Science and Technology 15(18): 857-868.
[4] Ayéna, A.C., Anani, K., Dosseh, K., Agbonon, A., Gbeassor, M. (2021): Comparative Study of Antimicrobial, Anti‐Inflammatory, and Antioxidant Activities of Different Parts from Pterocarpus Santalinoides l’Her. Ex. DC (Fabaceae). – Evidence‐Based Complementary and Alternative Medicine 7p.
[5] Bulle, S., Reddyvari, H., Nallanchakravarthula, V., Vaddi, D.R. (2016): Therapeutic potential of Pterocarpus santalinus L.: an update. – Pharmacognosy Reviews 10(19): 7p.
[6] Cai, Y., Yang, F., Huang, X. (2024): Oxidative stress and acute pancreatitis. – Biomedical Reports 21(2): 7p.
[7] Eddaikra, A., Eddaikra, N. (2021): Endogenous enzymatic antioxidant defense and pathologies. In Antioxidants-benefits, sources, mechanisms of action. – IntechOpen 59p.
[8] Ganiyev, A. (2024): Biochemical and Morphological Changes in Acute Pancreatitis and Diabetes: A Comparative Overview. – SYNAPSES: Insights Across the Disciplines 1(2): 31-37.
[9] Liu, M., Liu, C., Zhaxi, P., Kou, X., Liu, Y., Xue, Z. (2025): Research progress on hypoglycemic effects and molecular mechanisms of flavonoids: A review. – Antioxidants 14(4): 33p.
[10] Ma, D., Jiang, P., Jiang, Y., Li, H., Zhang, D. (2021): Effects of lipid peroxidation‐mediated ferroptosis on severe acute pancreatitis‐induced intestinal barrier injury and bacterial translocation. – Oxidative Medicine and Cellular Longevity 12p.
[11] Martín, M. Á., Ramos, S. (2021): Dietary flavonoids and insulin signaling in diabetes and obesity. – Cells 10(6): 22p.
[12] Mihoc, T., Latcu, S.C., Secasan, C.C., Dema, V., Cumpanas, A.A., Selaru, M., Pirvu, C.A., Valceanu, A.P., Zara, F., Dumitru, C.S., Novacescu, D. (2024): Pancreatic morphology, immunology, and the pathogenesis of acute pancreatitis. – Biomedicines 12(11): 38p.
[13] Mou, S.S., Tuhin, M.S.H., Muhit, M.A., Ahmed, F. (2025): Antioxidant, Cytotoxic, and Antimicrobial Activities of Pterocarpus santalinus Leaves. – Biomedical and Pharmacology Journal 18(1): 939-946.
[14] Muhammad, I., Rahman, N., Nishan, U., Shah, M. (2021): Antidiabetic activities of alkaloids isolated from medicinal plants. – Brazilian Journal of Pharmaceutical Sciences 57: 14p.
[15] Nagy-Pénzes, M., Hajnády, Z., Regdon, Z., Demény, M.Á., Kovács, K., El-Hamoly, T., Maléth, J., Hegyi, P., Hegedűs, C., Virág, L. (2022): Tricetin reduces inflammation and acinar cell injury in cerulein-induced acute pancreatitis: the role of oxidative stress-induced DNA damage signaling. – Biomedicines 10(6): 20p.
[16] Pant, P., Pandey, S., Dall'Acqua, S. (2021): The influence of environmental conditions on secondary metabolites in medicinal plants: A literature review. – Chemistry & Biodiversity 18(11): 14p.
[17] Pate, J.S., Jeschke, W.D. (1995): Role of stems in transport, storage, and circulation of ions and metabolites by the whole plant. – In Plant Stems, Academic Press 177p.
[18] Sharma, T., Gamit, R., Acharya, R., Shukla, V.J. (2021): Quantitative estimation of total tannin, alkaloid, phenolic, and flavonoid content of the root, leaf, and whole plant of Byttneria herbacea Roxb. – AYU (An International Quarterly Journal of Research in Ayurveda) 42(3): 143-147.
[19] Sok Yen, F., Shu Qin, C., Tan Shi Xuan, S., Jia Ying, P., Yi Le, H., Darmarajan, T., Gunasekaran, B., Salvamani, S., (2021): Hypoglycemic effects of plant flavonoids: a review. – Evidence‐Based Complementary and Alternative Medicine 12p.
[20] Szatmary, P., Grammatikopoulos, T., Cai, W., Huang, W., Mukherjee, R., Halloran, C., Beyer, G., Sutton, R. (2022): Acute pancreatitis: diagnosis and treatment. – Drugs 82(12): 1251-1276.
[21] Tang, Y., Sun, M., Liu, Z. (2022): Phytochemicals with protective effects against acute pancreatitis: a review of recent literature. – Pharmaceutical Biology 60(1): 479-490.
[22] Werawatganon, D., Vivatvakin, S., Somanawat, K., Tumwasorn, S., Klaikeaw, N., Siriviriyakul, P., Chayanupatkul, M. (2023): Effects of probiotics on pancreatic inflammation and intestinal integrity in mice with acute pancreatitis. – BMC Complementary Medicine and Therapies 23(1): 11p.
[23] Woo, W.S., Chi, H.J., YunChoi, H.S. (1977): Alkaloid screening of some Saudi Arabian plants. – Korean Journal of Pharmacognosy 8(3): 109-113.
[24] Xia, C.C., Chen, H.T., Deng, H., Huang, Y.T., Xu, G.Q. (2024): Reactive oxygen species and oxidative stress in acute pancreatitis: pathogenesis and new therapeutic interventions. – World Journal of Gastroenterology 30(45): 11p.
[25] Zhishen, J., Mengcheng, T., Jianming, W. (1999): The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. – Food Chemistry 64(4): 555-559.
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